ABSTRACT
Charismatic therapeutic potential of curcumin in biological research have triggered an interest to explore the thermal degradation pattern of curcumin, formation of ferulic acid and vanillin as degraded metabolites at different temperatures in methanol and corn oil. The results revealed 47% w/w loss of curcumin along with formation of 17% w/w vanillin and 9% w/w ferulic acid at boiling temperature of methanol while oil samples show 38.9% w/w loss of curcumin but not confirming the formation of ferulic acid and vanillin. Findings of this study revealed that formation of vanillin in methanol starts around 50[degree]C and its concentration goes on increasing up to 70[degree]C and then further degraded at boiling temperature of methanol. Formation of ferulic acid begins around 60[degree]C and initially increases with rise of temperature and then decreases approaching boiling point of methanol. Vanillin as well as ferulic acid was absent in corn oil samples though degradation of curcumin was observed by reduction in peak area of curcumin. The present study was done by applying a validated high-performance thin-layer chromatography method. The method involved glass-backed HPTLC plates precoated with silica gel 60F254 as the stationary phase and toluene: ethyl acetate: methanol [8:1:1, v/v/v] as mobile phase
ABSTRACT
In the present study an analytical method of high-performance thin-layer chromatography [HPTLC] has been developed for quantification of glycyrrhizin for marketed antistress liquorice root capsules [LRC] and herbal tea [HT]. Chromatography was performed by using mobile phase ethyl acetate [EA]: glacial acetic acid [GAA]: Methanol [MeOH]: water [H2O] in proportion of 6:2:2:1, v/v/v/v. The developed plate was scanned and quantified densitometrically at absorption maxima 254nm. The method was validated for various analytical parameters viz. precision, accuracy, recovery, robustness, specificity, detection and quantification limits. The developed system was found to give compact spot for glycyrrhizin [Rf = 0.33 +/- 0.001]. The linearity relationship was described by the equation Y=6.841X+ 70.428. The limit of detection [34 ng band-1], limit of quantification [101ng band-1], recovery [99.4-99.8%], and precision [
ABSTRACT
Biomarker rutin was analyzed in methanol extracts of leaves of five different species of genus Ficus [Ficus carica, Ficus nitida, Ficus ingens, Ficus palmata and Ficus vasta] by NP-HPTLC [Method I] and RP- HPTLC methods [Method II]. The development and validation for method I was carried out with silica gel 60F[254] plates using EA: GAA: FA: H[2]O [10:1:1:2.5, v/v/v/v] as developing system. Method II was carried out on silica gel 60F[254] RP-18 plates using mobile phase ACN: H[2]O [4:6 v/v]. Both analyses were scanned at 305 nm and were found to give well resolved peak of rutin at Rf 0.28 +/- 0.01 and 0.68 +/- 0.03 for Method I and Method II, respectively. The percentage of rutin was found to be 0.51% and 0.66% in F. ingens, 0.24% and 0.54% in F. palmata and 0.14% and 0.17% in F. vasta by Method I and Method II, respectively. Method II [RP-HPTLC] was found to be more accurate, precise and sensitive than Method I. Method II can be used as an important tool for standardization and quality control of bulk drugs and in-process formulations of rutin
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Investigations for anti-inflammatory potential and categorization of Sudanese medicinal plants according to their potency. Anti-inflammatory effect of plants' extracts of 17 genera were studied using the carrageenan induced inflammation in rats' paws. The plant extracts were obtained using methanol and dichloromethane as solvent and administered intra peritoneally at the concentration of 2g/kg body weight. The results obtained in this experiment strongly support and validate the traditional uses of these Sudanese medicinal plants to treat various inflammatory diseases. 63.9% of plants extracts showed marked inhibition of inflammation induced by carrageenan [78.3% out of this percentage represented by methanolic extract], 27.8% showed no activity and 8.3% enhanced the carrageenan induced inflammation. The anti-inflammatory effect of many of these plants has not been reported previously, yet they have been extensively used in Sudanese folkloric medicine. The result of this study justify the traditional medicinal use of the evaluated plants species in treating inflammatory disorders and helped in categorizing the investigated plants into most useful, moderately useful and least useful category for inflammatory diseases. Out of the 17 investigated plant species 05 belongs to most useful and 06 belongs to moderately useful category. However, toxicity studies are required to prove the safety of these plant materials
Subject(s)
Animals, Laboratory , Plants, Medicinal , Plant Extracts , Rats, Wistar , InflammationABSTRACT
<p><b>OBJECTIVE</b>To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L. (R. vesicarius).</p><p><b>METHODS</b>In the present study, acetone, ethyl acetate, n-butanol, and methanol extracts of R. vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate, DNA sugar damage, scavenging of hydrogen peroxide, diphenylphosphine DPPH radical scavenging activity, total phenolic content, total flavonoids content and total proanthocyanidin. High performance thin layer chromatography finger print profiling of R. vesicarius L. was also done.</p><p><b>RESULTS</b>Lipid peroxidation induced by the iron/ascorbate system, hydrogen peroxide, diphenylphosphine and DNA sugar damage were inhibited by the addition of different extract of R. vesicarius. Among them, methanolic extract showed maximum efficacy. The methanolic extract showed the highest total phenolic, total flavonoids and total proanthocyanidin contents.</p><p><b>CONCLUSIONS</b>The results suggest that the extracts can be a vital source of phytochemical antioxidants.</p>
ABSTRACT
Objective: To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L. (R. vesicarius). Methods: In the present study, acetone, ethyl acetate, n-butanol, and methanol extracts of R. vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(